Reversible Inactivation and Dissociation of Yeast Hexokinase
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چکیده
منابع مشابه
Reversible inactivation and dissociation of yeast hexokinase.
Recent’ work on the reactivation and reconstitution of several denatured enzymes has provided increasing understanding of the process of reversible protein denaturation. Studies on ribonuclease by Anfinsen et al. (1) and White (2) have convincingly demonstrated that under favorable conditions a complet,ely denatured protein can resume the active three-dimensional configuration of the native mol...
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Formyltetrahydrofolate synthetase undergoes inactivation at pH values below 7. Inactivation is accompanied by both the dissociation of the tetramer into monomers and the exposure of aromatic amino acids to the solvent. The rate of inactivation is a function of pH. This rate and the rate of exposure of tryptophan residues to the aqueous environment follow first order kinetics. After prolonged in...
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The rate of the ATP:glucose transphosphorylase reaction catalyzed by yeast hexokinase, isozyme PII, is inhibited by lowering the pH of the reaction below 7.0, especially at suboptimal concentrations of ATP. This effect of acidity is largely overcome by activators such as orthophosphate, citrate, malate, 3-phosphoglycerate, and riboside triphosphates. Thus, in the acid range, ATP appears to serv...
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Yeast hexokinase, a homodimer (100 kDa), is an important enzyme in the glycolytic pathway. Although Cibacron Blue 3G-A (Reactive Blue 2) has been previously shown to inactivate yeast hexokinase, no comprehensive study exists concerning the nature of interaction(s) between hexokinase and the blue dye. A comparison of the computer-generated three-dimensional (3D) representations showed considerab...
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Activation of yeast hexokinase PI1 at low pH by citrate or ATP has been shown to occur at the monomer level, without requiring association to the dimer level. Thus, the previous suggestion (Steitz, T. A., Anderson, W. F., Fletterick, R. G., and Anderson, C. M. (1977) J. Biol. Chem. 252, 4494-4500) that anionic activators of hexokinase, such as citrate and ATP, act by binding to the intersubunit...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96994-2